Type I signal peptidase and protein secretion in S. epidermidis

M.E. Powers, P.A. Smith, T.C. Roberts, B.J. Fowler, C.C. King, S.A. Trauger, G. Siuzdak, F.E. Romesberg, J. Bacteriol. (2011) 193:340-348.
pubpic2011powersWe describe the use of an arylomycin derivative, along with two-dimensional gel electrophoresis and LC-MS/MS, to identify eleven proteins whose secretion from stationary phase S. epidermidis is dependent on SPase activity. The data also suggest that the arylomycins should be valuable chemical biology tools for the study of protein secretion in a wide variety of different bacteria