R.A. Rodriguez, D.B. Steed, Y. Kawamata, S. Su, P.A. Smith, T.C. Steed, F.E. Romesberg, P.S. Baran, J. Am. Chem. Soc. (2014) 136:15403-15413.
In this collaborative work with the Baran Lab, we show that the axinellamines have promising activity against Gram-positive and Gram-negative bacteria, suggesting that their scaffold has the potential for further development. Details regarding their mode of action remain to be elucidated, but the axinellamines appear to cause secondary membrane destabilization and may inhibit normal septum formation..
R. Adhikary, J. Zimmermann, J. Liu, R. Forrest, T. Janicki, P. Dawson, S. Corcelli, F.E. Romesberg, J. Am. Chem. Soc. (2014) 136:13474-13477.
We characterize the IR stretching frequencies of deuterated variants of proline and four proline residues of an Src homology 3 domain protein. CD stretching frequencies are shifted to lower energies due to hyperconjugation with Ni electron density, and along with DFT calculations, the data reveal that the Ni+1-H–Ni interactions constitute H-bonds that may play an important role in protein folding, structure, and function.
K. Dhami, D.A. Malyshev, P. Ordoukhanian, T. Kubelka, M. Hocek, F.E. Romesberg, Nucleic Acids Res (2014) 42:10235-10244.
We report a PCR-based screen to identify promising unnatural base pairs from a library of 111 unnatural nucleotides (~6,000 candidate base pairs).
D.A. Malyshev, K. Dhami, T. Lavergne, T. Chen, N. Dai, J.M. Foster, I.R. Corrêa, F.E. Romesberg, Nature (2014) 509:385-388.
We demonstrate that E. coli can stably maintain a plasmid containing d5SICS–dNaM with only the addition of an exogenously expressed algal nucleotide triphosphate transporter. Read the associated News & Views or read more in The New York Times, The Atlantic, Wired, Science, Chemistry World, and The San Diego Union Tribune. This work was also recognized as one of the top 10 scientific achievements of 2014 by Science.
R. Adhikary, J. Zimmermann, P.E. Dawson, F.E. Romesberg, ChemPhysChem (2014) 15:849-853.
We use IR spectroscopy to characterize nSH3 variants labeled with CN or N3 at five different positions. Like carbon-deuterium (C-D) bonds, CN and N3 can provide information about their surrounding protein environment, but unlike C-D, they tend to be perturbative and thus should be used with caution.
L. Li, M. Degardin, T. Lavergne, D. Malyshev, K. Dhami, P. Ordoukhanian, F.E. Romesberg, J. Am. Chem. Soc. (2014) 136:826-829.
Cover Story In this work, we identify dTPT3-dNaM, which is PCR amplified with a natural base pair-like efficiency and fidelity. We also show that the dTPT3 scaffold is uniquely tolerant of attaching a propargyl amine linker, enabling site-specific labeling of PCR-amplified DNA.
T. Chen, F.E. Romesberg, FEBS Lett. (2014) 588:219-229.
We review directed evolution methods used to tailor the properties and/or substrate repertoire of polymerases for different applications and provide a survey of the many polymerases that have been evolved.