F.-Y. Dupradeau, D.A. Case, C. Yu, R. Jimenez, F.E. Romesberg, J. Am. Chem. Soc. (2005) 127:15612-15617.
We show that when HBO is positioned in the minor groove of DNA, the enol tautomer of HBO is preferentially stabilized, whereas our previous study showed that when HBO is positioned in the major groove, the keto tautomer is preferentially stabilized. Based on MD simulations, we suggest that this results from the formation of a stable hydrogen-bond between the HBO enol and an H-bond acceptor that is only available in the minor groove.
A.M. Leconte, L.Chen, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2005) 127:12470-12471.
We report the use of an activity-based selection system to evolve a DNA polymerase that more efficiently replicates DNA containing the PICS self-pair.
A.A. Henry and F.E. Romesberg, Curr. Opin. Biotechnol. (2005) 16:370-377.
We review several activity-based screening and selection approaches that have been used to identify polymerases with novel activities.
R.T. Cirz, J.K. Chin, D.R. Andes, V. de Crécy-Lagard, W.A. Craig, F.E. Romesberg, PLoS Biol. (2005) 3:e176.
The results presented herein indicate that the inhibition of mutation could serve as a novel therapeutic strategy to combat the evolution of antibiotic resistance. Read more in C&EN, C&EN 2005 highlights, and Nat. Rev. Genetics, or listen to Sci. Am. Science Talk.
R.C. Holmberg, A.A. Henry, F.E. Romesberg, Biomol. Eng. (2005) 22:39-49.
We review screening and selection approaches, both in vivo and in vitro, that have been used to evolve polymerases with a variety of novel properties, such as thermal stability, resistance to inhibitors, and altered substrate specificity.