Y. Wu, M. Fa, E.L. Tae, P.G. Schultz, F.E. Romesberg, J. Am. Chem. Soc. (2002) 124:14626-14630.
We report our initial efforts toward the development of an unnatural in vivo nucleoside phosphorylation pathway that is based on nucleoside salvage enzymes.
R. Jimenez, F.E. Romesberg, J. Phys. Chem. B. (2002) 106(35):9172-9180.
Transient absorption, transient grating, and 3PEPS measurements are used to characterize the photophysics of the heme chromophore in the folded protein and in two different unfolded proteins. We find that cyt c has little structural heterogeneity in the folded state, and a larger degree of structural heterogeneity in the unfolded states that depends on the unfolding conditions.
C. Yu, A.A. Henry, F.E. Romesberg, P.G. Schultz, Angew. Chem. Int. Ed. Engl. (2002) 41:3841-3844.
Heteroatom substitution of ICS via replacement of C6 with nitrogen and thio substitution at C10 provides the base SNICS, which forms stable self-pairs and can therefore be used for efficient unnatural base pair replication.
F.E. Romesberg, C. Yu, S. Matsuda, A.A. Henry, Curr. Prot. Nucleic Acid Chem. (2002) 10:1.5:1.5.1–1.5.36..
We describe methods for the synthesis and characterization of modified nucleobases.
D. Hanway, J.K. Chin, G. Xia, G. Oshiro, E.A. Winzeler, F.E. Romesberg, Proc. Natl. Acad. Sci. USA (2002) 99:10605-10610.
A competitive growth assay is used to identify yeast genes involved in the repair of UV- or MMS-induced DNA damage using a collection of 2,827 yeast strains in which each strain has a single ORF replaced with a cassette containing two unique sequence tags. Identified genes include three uncharacterized ORFs, as well as genes that encode protein products implicated in ubiquitination, gene silencing, and transport across the mitochondrial membrane.
G. Xia, L. Chen, T. Sera, M. Fa, P.G. Schultz, F.E. Romesberg, Proc. Natl. Acad. Sci. USA (2002) 99:6597-6602.
We use an activity-based phage display method to screen a Stoffel fragment (SF) polymerase library, and we identify three SF mutants that incorporate ribonucleoside triphosphates virtually as efficiently as the wild-type SF polymerase incorporates dNTP substrates.
O.K. Abou-Zied, R. Jimenez, E.H.Z. Thompson, D.P. Millar, F.E. Romesberg, J. Phys. Chem. A. (2002) 106:3665–3672.
The solvent-dependent ground-state conformational equilibrium and excited-state dynamics of 2-(2‘-hydroxyphenyl)benzoxazole are characterized in several solvents on the femtosecond to nanosecond time scales.