Efforts toward expansion of the genetic alphabet: replication of DNA with three base pairs

E.L. Tae, Y. Wu, G. Xia, P.G. Schultz, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2001) 123:7439-7440.
pubpic2001taeWe show that the unnatural self-pair formed between two 7-azaindole nucleosides can be replicated in DNA using binary polymerase system – KF efficiently synthesizes the self-pair, and pol β efficiently extends it.


Tautomerization dynamics of a model base pair in DNA

O.K. Abou-Zied, R. Jimenez, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2001) 123:4613–4614.
pubpic2001abouziedHBO was synthesized and incorporated into DNA oligonucleotides and each oligonucleotide was hybridized to a complementary oligonucleotide containing an abasic site at the position opposite HBO. Analysis of steady state emission spectra of the oligonucleotides indicates that the DNA environment selectively stabilizes the proton-transfer product tautomer of HBO, suggesting that flanking bases may be strongly coupled electronically.


Direct observation of protein vibrations by selective incorporation of spectroscopically observable carbon-deuterium bonds in cytochrome c

J.K. Chin, R. Jimenez, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2001) 123:2426-2427.
pubpic2001chinWe introduce C−D bonds into horse heart cyt c and use the C−D stretching vibration, which absorbs at approximately 2100 cm-1, as a spectroscopic probe of the protein environment.