Efforts toward expansion of the genetic alphabet: DNA polymerase recognition of a highly stable, self-pairing hydrophobic base
D.L. McMinn, A.K. Ogawa, Y. Wu, J. Liu, P.G. Schultz, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (1999) 121:11585-11586.
This study demonstrates that hydrophobicity is a sufficient driving force for the stable and selective pairing of the 7-propynyl isocarbostyril nucleoside in duplex DNA, as well as for the selective enzymatic incorporation of this nucleobase against itself during DNA synthesis.