Polymerase chain transcription: exponential synthesis of RNA and modified RNA

T. Chen, F.E. Romesberg, J Am Chem Soc (2017) Published online July 17.
pubpic2017chenWe show that the evolved Stoffel fragment mutant, SFM4-3, efficiently transcribes RNA or 2′-F-modified RNA and that it also efficiently PCR amplifies oligonucleotides of mixed RNA and DNA composition. Using thermocycling and a novel DNA template, we demonstrate a polymerase chain transcription (PCT) reaction that results in the exponential production of orders of magnitude more RNA or modified RNA than is available by conventional transcription.