Synthetic biology parts for the storage of increased genetic information in cells

S.E. Morris, A.W. Feldman, F.E. Romesberg, ACS Synth Biol (2017) Published online June 27.
pubpic2017MorrisWe report a steady-state kinetic characterization of the rate with which the Klenow fragment of E. coli DNA polymerase I synthesizes the dNaM-dTPT3 UBP and its mispairs in a variety of sequence contexts. The data demonstrate that dNaMTP and dTPT3TP are well optimized and standardized parts for the expansion of the genetic alphabet.