Natural-like replication of an unnatural base pair for the expansion of the genetic alphabet and biotechnology applications

L. Li, M. Degardin, T. Lavergne, D. Malyshev, K. Dhami, P. Ordoukhanian, F.E. Romesberg, J. Am. Chem. Soc. (2014) 136:826-829.
pubpic2013bliCover Story In this work, we identify dTPT3-dNaM, which is PCR amplified with a natural base pair-like efficiency and fidelity. We also show that the dTPT3 scaffold is uniquely tolerant of attaching a propargyl amine linker, enabling site-specific labeling of PCR-amplified DNA.