Structural insights into DNA replication without hydrogen bonds

K. Betz, D.A. Malyshev, T. Lavergne, W. Welte, K. Diederichs, F.E. Romesberg, A. Marx, J. Am. Chem. Soc. (2013) 135:18637-18643.
pubpic2013betzWe report the structural characterization of the prechemistry complexes with KlenTaq polymerase corresponding to the insertion of dNaMTP opposite d5SICS, as well as mulitple postchemistry complexes in which the already formed unnatural base pair is positioned at the postinsertion site.