Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic alphabet

D.A. Malyshev, K. Dhami, H.T. Quach, T. Laveryne, P. Ordoukhanian, A. Torkamani, F.E. Romesberg, Proc. Natl. Acad. Sci. USA (2012) 109:12005-12010.
pubpic2012malyshevWe examine the PCR amplification of DNA containing one or more d5SICS-dNaM pairs and demonstrate that this DNA may be amplified with high efficiency and with greater than 99.9% fidelity. The results demonstrate that for PCR and PCR-based applications, d5SICS-ddNaM is functionally equivalent to a natural base pair, and when combined with dA-dT and dG-dC, provides the first fully functional six-letter genetic alphabet. Read more in Nature and Biotechniques.