Yeasts acquire resistance secondary to antifungal drug treatment by adaptive mutagenesis

D. Quinto-Alemany, A. Canerina-Amaro, L.G. Hernández-Abad, F. Machín, F.E. Romesberg, C. Gil-Lamaignere, PLoS ONE (2012) 7:e42279.
pubpic2012quinto_alemanyStressful lifestyle associated mutation (SLAM) assays are used to demonstrate that both S. cerevisiae and C. albicans undergo mutations to acquire resistance to the antifungal agents 5-fluorocytosine and caspofungin. Examination of the mutation spectrum shows that these aquired mutations are different from those that the occur spontaneously, in the absence of antifungal treatment. Overall, the work present a much needed model system for studying adaptive mutagenesis in eukaryotes.