Solution structure, mechanism of replication, and optimization of an unnatural base pair

D.A. Malyshev, D.A. Pfaff, S.I. Ippoliti, G.T. Hwang, T.J. Dwyer, F.E. Romesberg, Chem. Eur. J. (2010) 16:12650-12659.
pubpic2010malyshevWe synthesize a variety of derivatized unnatural base pairs and characterize their structures and DNA polymerase-mediated replication. We also present further evidence supporting an intercalation-based mechanism of replication, and show that dDMO—d5SICS represents significant progresstoward the expansion of the genetic alphabet.