Directed evolution of DNA polymerases for next generation sequencing

A.M. Leconte, M.P. Patel, L.E. Sass, P. McInerney, M. Jarosz, L. Kung, J.L. Bowers, P.R. Buzby, J.W. Efcavitch, F.E. Romesberg, Angew. Chem. Int. Ed. (2010) 34:5921-5924.
pubpic2010xxWe identified a mutant of Taq DNA polymerase that incorporates fluorophore-labeled substrates 50- to 400-fold more efficiently into scarred primers in solution and also demonstrates significantly improved performance under actual sequencing conditions.