Major groove derivatization of an unnatural base pair

Y.J. Seo, F.E. Romesberg, ChemBioChem (2009) 10:2394-2400.
Cover Story We synthesize and evaluate several dMMO2 derivatives with meta-chlorine, -bromine, -iodine, -methyl, or -propinyl substituents. Complete characterization of unnatural base pair and mispair synthesis and extension reveal that the modifications have large effects only on the efficiency of unnatural base pair synthesis and that the effects likely result from a combination of changes in steric interactions, polarity, and polarizability.