The effects of unnatural base pairs and mispairs on DNA duplex stability and solvation

G.T. Hwang , Y. Hari, F.E. Romesberg, Nucleic Acids Res, (2009) 37:4757-4763.
pubpic2009hwangWeexamine six pyridine-based nucleotides, differentiated by methyl substitution, that are designed to vary both inter- and intra-strand packing within duplex DNA. We show that incorporation of one of these nucleobases, d34DMPy, as a self pair or as a mispair with dA significantly increases oligonucleotide hybridization fidelity at other positions within the duplex.