Transcription of an expanded genetic alphabet

Y.J. Seo, S. Matsuda, F.E. Romesberg, J. Am. Chem. Soc. (2009) 131:5046-5047.
pubpic2009seoWe show that both of the unnatural base pairs d5SICS:dMMO2 and d5SICS:dNaM are selectively transcribed by T7 RNA polymerase and that the efficiency of d5SICS:dNaM transcription in both possible strand contexts is only marginally reduced relative to that of a natural base pair.

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