The determinants of stability and folding in evolutionarily diverged cytochromes c

M.C. Thielges, J. Zimmermann, P.E. Dawson, F.E. Romesberg, J. Mol. Biol. (2009) 24:159-167.
pubpic2009thielgesUsing site-selectively incorporated carbon–deuterium bonds, we show that like equine cyt c, bovine cyt c is induced to unfold by guanidine hydrochloride via a stepwise mechanism, but it does not populate an intermediate as is observed with the equine protein.