Optimization of an unnatural base pair towards natural-like replication

Y.J. Seo, G.T. Hwang, P. Ordoukhanian, F.E. Romesberg, J. Am. Chem. Soc. (2009) 131:3246-3252.
pubpic2009seoTo better understand and optimize the slowest step of replication of the unnatural base pair formed between the nucleotides dMMO2 and d5SICS, the insertion of MMO2 opposite d5SICS, we synthesize two dMMO2 derivatives, d5FM and dNaM, which differ from the parent nucleobase in terms of shape, hydrophobicity, and polarizability, and we characterize their enzymatic replication.