Unnatural substrate repertoire of A, B, and X family DNA polymerases

G.T. Hwang, F.E. Romesberg, J. Am. Chem. Soc. (2008) 130:14872-14882.
pubpic2008hwangWe examine the ability of five different polymerases (family A polymerases from bacteriophage T7 and Thermus aquaticus, family B polymerases from Thermococcus litoralis and Thermococcus 9°N-7, and the family X polymerase, human polymerase β) to replicate DNA containing four different unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs, and we identify some aspects of recognition that are conserved.