Optimization of the pyridyl nucleobase scaffold for polymerase recognition and unnatural base pair replication

Y. Hari, G.T. Hwang, A.M. Leconte, N. Joubert, M. Hocek, F.E. Romesberg, ChemBioChem, (2008) 9:2796-2799.
pubpic2008hariWe demonstrate that the pyridyl nucleobase scaffold can be optimized for replication, both as a self pair and as a component of a heteropair, and we identify a pyridyl-nucleotide self pair that is well recognized by a DNA polymerase, allowing it to be replicated and used to synthesize site-specifically labeled DNA in good yields.