Carbon-Deuterium bonds as probes of dihydrofolate reductase

M.C. Thielges, D.A. Case, F.E. Romesberg, J. Am. Chem. Soc. (2008) 130:6597-6603.
pubpic2008thielgesWe report a preliminary analysis of (methyl-d3) methionine residues, Met16, Met20, and Met42, within DHFR. The results confirm the sensitivity of the carbon−deuterium bonds to their local protein environment and demonstrate that dihydrofolate reductase is electrostatically and dynamically heterogeneous.