Discovery, characterization, and optimization of an unnatural base pair for expansion of the genetic alphabet

A.M. Leconte, G.T. Hwang, S. Matsuda, P. Capek, Y. Hari, F.E. Romesberg, J. Am. Chem. Soc. (2008) 130:2336-2343.
pubpic2008leconteWe identify the UBP dSICS:dMMO2 by screening a pool of 3600 candidate base pairs. From the results of these studies, we designed an optimized base pair, d5SICS:dMMO2, which is efficiently and selectively synthesized by Kf within the context of natural DNA.  Read more in New Scientist, JAMA, and Popular Science.