Polymerase recognition and stability of fluoro substituted pyridone nucleobase analogs

G.T. Hwang, A.M. Leconte, F.E. Romesberg, ChemBioChem. (2007) 8:1606-1611.
pubpic2007hwangWe synthesize and characterize three fluoro-substituted pyridone nucleoside analogues. Generally, we find that the specific fluorine substitution pattern of the analogs has little impact on unnatural pair or mispair stability, but does significantly increase the rate at which the pyridone-based unnatural base pairs are extended.