Minor groove hydrogen bonds and the replication of unnatural base pairs

S. Matsuda, A.M. Leconte, F.E. Romesberg, J. Am. Chem. Soc. (2007) 129:5551-5557.
pubpic2007matsudaWe examine the enzymatic synthesis of DNA with six different unnatural nucleotides bearing methoxy-derivatized nucleobase analogues. Different nucleobase substitution patterns were used to systematically alter the nucleobase electronics, sterics, and hydrogen-bonding potential. Our results indicate that ortho methoxy groups should be generally useful for the effort to expand the genetic alphabet.