Substituent effects on the pairing and polymerase recognition of unnatural base pairs

G.T. Hwang, F.E. Romesberg, Nucleic Acids Res. (2006) 34:2037-2045.
pubpic2006hwangWe report the synthesis, stability and polymerase recognition of nucleoside analogs bearing single bromo- or cyano-derivatized phenyl rings and find that both modifications generally stabilize base pair formation to a greater extent than methyl or fluoro substitution.