Directed evolution of novel polymerases

R.C. Holmberg, A.A. Henry, F.E. Romesberg, Biomol. Eng. (2005) 22:39-49.
pubpic2005xxWe review screening and selection approaches, both in vivo and in vitro, that have been used to evolve polymerases with a variety of novel properties, such as thermal stability, resistance to inhibitors, and altered substrate specificity.