Protein dynamics and the immunological evolution of molecular recognition

R. Jimenez, G. Salazar, J. Yin, T. Joo, F.E. Romesberg, Proc. Natl. Acad. Sci. USA (2004) 101:3803–3808.
pubpic2004jimenez3PEPS and steady-state spectroscopy in combination with binding and structural data, are used to examine the immunological evolution of protein flexibility in an anti-fluorescein antibody. We show that antibody dynamics are systematically manipulated during affinity maturation, and suggest that the evolution of protein flexibility may be a central component of the immune response.