Directed evolution of novel polymerase activities: mutation of a DNA polymerase into and efficient RNA polymerase
G. Xia, L. Chen, T. Sera, M. Fa, P.G. Schultz, F.E. Romesberg, Proc. Natl. Acad. Sci. USA (2002) 99:6597-6602.
We use an activity-based phage display method to screen a Stoffel fragment (SF) polymerase library, and we identify three SF mutants that incorporate ribonucleoside triphosphates virtually as efficiently as the wild-type SF polymerase incorporates dNTP substrates.