Tautomerization dynamics of a model base pair in DNA

O.K. Abou-Zied, R. Jimenez, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2001) 123:4613–4614.
pubpic2001abouziedHBO was synthesized and incorporated into DNA oligonucleotides and each oligonucleotide was hybridized to a complementary oligonucleotide containing an abasic site at the position opposite HBO. Analysis of steady state emission spectra of the oligonucleotides indicates that the DNA environment selectively stabilizes the proton-transfer product tautomer of HBO, suggesting that flanking bases may be strongly coupled electronically.