Direct observation of protein vibrations by selective incorporation of spectroscopically observable carbon-deuterium bonds in cytochrome c
J.K. Chin, R. Jimenez, F.E. Romesberg, J. Am. Chem. Soc. (Comm.) (2001) 123:2426-2427.
We introduce C−D bonds into horse heart cyt c and use the C−D stretching vibration, which absorbs at approximately 2100 cm-1, as a spectroscopic probe of the protein environment.